MyTREC    Real-Time qPCR Assay Kit Specifications  (Singleplex / Human) 
 
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  • GP-T10120100 / GP-A20120100                  100 x 20 ul Reactions
  • GP-T10120500 / GP-A20120500                  500 x 20 ul Reactions
 
 
Components                                                                                                        
  • Calibrators
  • Gene Expression Master Mix / ROX   reference dye stock solution, separate                                                                              
  • Primers / Probe 
  • Reaction Enhancer 
  • Nuclease-Free Water
  • Positive Control (TREC or Beta Actin positive)
 
Storage and Stability
All components of the kit should be stored at -20 to -30 deg C. Avoid repeat freeze-thaws. Avoid exposure of components [probes, ROX,  Gene Expression Master Mix (after ROX   is added)] to light. With proper storage and handling, the full activity of the kit is retained until the expiry date.
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Shipping
The product is shipped with ice/gel packs.
 
Limit Of Quantification 
  • MyTREC    TREC Assay Kit            GP-T10120100 / GP-T10120500 - detects 25 genome equivalent copies of TRECs
  • MyTREC    Beta Actin Assay Kit    GP-A20120100 / GP-A20120500 - detects 100 genome equivalent copies of Beta Actin 
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Features
  • Specific, sensitive - The Calibrators have been designed and optimized with TaqMan    dual labeled fluorescent probes for an efficient and accurate quantification of TRECs                                                                           
  • Complete, All-In-One, Ready-To-Use - The MyTREC    Kit is "complete" containing all the components necessary to run the Real-Time qPCR Assay.                                                                        
  • Linear dynamic range - Broad range of Calibrators provides reliable testing of precious samples                                                                  
  • Absolute Quantification - By generating an Absolute Calibration / Standard curve using Real Time qPCR Assay.                                                                   
Instrument Compatibility
The MyTREC    Kit, among other components contains a separate ROX   stock aliquot and reporter dyes labeled with FAM / VIC making it compatible with all commonly used Real-Time PCR Instruments. Determine if your instrument is a High / Low / No ROX instrument and add the ROX   stock reference dye to the Gene Expression Master Mix, according to the specifications detailed in the product brochure. Also, most High / Low ROX   instruments are capable of analyzing the Real-Time data with the passive reference dye signal either on or off. See "Resources" for know more about instrument compatibility.
Description
The MyTREC    Real Time qPCR Assay Reagent Kit has been designed and developed for sensitive and accurate quantification of Human TRECs in a blood volume as little as 1 ul. The proprietary Calibrators are "Ready-To-Use" and are serially diluted in a stabilizing diluent. The kit is formulated with TaqMan   dual-labeled fluorescent probes and a Gene Expression Master Mix containing antibody-mediated hot-start DNA Polymerase, Enhancers, Stabilizers, dNTPs. A separate ROX   reference dye stock aliquot is provided for optional use and to enable instrument compatibility. The kit is optimized for standard mode on fast qPCR instruments and standard cycling conditions on standard qPCR machines.
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